A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Ceftazidime (CTZ) and Clavulanic acid (CA) by employing first order derivative zero crossing method in dist.water. The first order derivative absorption at 256 nm (zero cross point of Ceftazidime) was used for quantification of Clavulanic acid and 278 nm (zero cross point of Clavulanic acid) for quantification of Ceftazidime. The linearity was established over the concentration range of 4-20 µg/ml and 50-250 µg/ml for Ceftazidime and Clavulanic acid with correlation coefficient r2 0.9974 and 0.9979, respectively. The mean % recoveries were found to be in the range of 99.63%–99.88% and 99.96%–100.29% for Ceftazidime and Clavulanic acid, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of pharmaceutical dosage form.
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